You examine a bacterial gene that produces a small peptide. The DNA sequence is

Question

You examine a bacterial gene that produces a small peptide. The DNA sequence is predicted to produce the mRNA sequence indicated below (Questions 13-15). 5?-UCUUAGGAGGUAUCUCCAUGUCCACUACGAGAGGAUUUUAAUGAUA-3? Shine-Dalgarno motif f Site of insertion for question 14 13) Predict the amino acid sequence of the peptide produced from this mRNA (use the 3-letter abbreviation for amino acid names; e.g. ILE for isoleucine, ALA for alanine. Look it up online if you can?t find it in one of your biology books). 14) What peptide sequence would be produced if you inserted an extra C nucleotide in the DNA (between C&A;, as shown in the figure above) (use the 3-letter abbreviation for amino acids)? 15) What would happen if you deleted the DNA region that encodes the Shine-Dalgarno motif? (What would it affect, transcription or translation? How?

Explanation / Answer

13) SER, stop codon, Glutamic acid, VAL, SER, SER, MET, SER, THR, THR, ARG, GLY, PHE, stop codon, stop codon

14) SER, stop codon, Glutamic acid, VAL, SER, ILE, HIS, VAL, HIS, TYR, Glutamic acid, ARG, ILE, stop codon, MET, ILE

15) If shine dalgarno motif is deleted, there will be change in the amino acid sequence. There will bw change in translation.

Related Questions

Navigate

• Browse (All)
• Browse Y
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.