What will be the ratio of k\'eq ( ph=7.8) K\'eq( ph=7.0) for the cytoplasmic LDH

Question

What will be the ratio of k'eq ( ph=7.8) K'eq( ph=7.0) for the cytoplasmic LDH?

So what is the ratio at ph 7.8

What is the ratio at ph 8.

EXPLAIN EXPLAIN

1. In the mitochondria there is an (R) - lactate dehydrogenase that, unlike the cytoplasmic enzyme, uses cytochrome c as an oxidizing agent and D - lactate as a substrate. The product, pyruvate, is the same for both enzymes. Given that the reduction potential of cytochrome c is 0.235 volts at pH = 7: Cyto - c[Fe^+3] + e^ - cyto -c [Fe^2] and that the reduction potential of pyruvate to D - lactate is identical to that of L - lactate What will be the ratio of k'eq ( ph=7.8) K'eq( ph=7.0) for the cytoplasmic LDH? So what is the ratio at ph 7.8 What is the ratio at ph 8. EXPLAIN EXPLAIN

Explanation / Answer

lactate + NAD+????? pyruvate + NADH + H+
The standard reduction potential at pH 7 for (NAD+/NADH) is -0.320 volts, and the standard
reduction potential at pH 7 for (pyruvate/lactate) is -0.185 volts.

?E'0 = E'0(red )? E'0(red )
?E'0 = ?0.320v ? (?0.185v) = ?0.135v

The negative value indicates an unfavorable reaction. The free energy change for this reaction is
given by the equation below where n is the number of electrons per mole transferred in this
reaction and F is Faraday

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