2. Atpenin is a relatively new antifungal antibiotic produced by Penicillium. In
ID: 37186 • Letter: 2
Question
2. Atpenin is a relatively new antifungal antibiotic produced by Penicillium. In order to investigate its mode of action the following experiments were preformed: A suspension of freshly-prepared mitochondria in phosphate buffer was incubated with various compounds and O2 consumption, measured with an oxygen electrode: When glutamate + malate is used instead of succinate, a big difference was found fig (b). Addittion of atepnin had NO EFFECT on the rate of O2 consuption. What does this suggest about the mode of action of atpenin? Can some exaplain besides the fact that it would act as an inhibitory... byt what does the fact that glutamate +malate have so what would the mode of action of atpenin be besides acting as inhititory.... on it. Expain please.Explanation / Answer
Atpenin inhibit production of oxygen and increase the demand for ATP derived from ADP by activating ATP dependent potassium channels in mitochiondria. The level of reactive oxygen species produced by mitochondria is significantly higher in presence of glutamate + malate than that of succinate. This influences the effect of inhibitor atpenin
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