A) Why is there a large increase in the rate of 02 consumption when 2,4 dinitrop
ID: 37216 • Letter: A
Question
A) Why is there a large increase in the rate of 02 consumption when 2,4 dinitrophenol added? && why?!?
B)) What would happen if DNP was added instead of ADP in fig A ..... ps.I get that DNP acts as an uncoupler? But what does that have to do with anything in this graph.. Like in the first one DNP is present rather than in the second one.. So what does the role have to do with anything in succinate vs effect of inibitor
Explain both please.
2. Atpenin is a relatively new antifungal antibiotic produced by Penicillium. In order to investigate its mode of action the following experiments were preformed: A suspension of freshly-prepared mitochondria in phosphate buffer was incubated with various compounds and O2 consumption, measured with an oxygen electrode: A) Why is there a large increase in the rate of 02 consumption when 2,4 dinitrophenol added? && why?!? B)) What would happen if DNP was added instead of ADP in fig A ..... ps.I get that DNP acts as an uncoupler? But what does that have to do with anything in this graph.. Like in the first one DNP is present rather than in the second one.. So what does the role have to do with anything in succinate vs effect of inibitor Explain both please.Explanation / Answer
A) In the presence of 2,4 dinitrophenol, electron transport from NADH to O2 proceeds in a normal fashion, but ATP is not formed by mitochondrial ATP synthase because the proton-motive force across the inner mitochondrial membrane is dissipated. This loss of respiratory control leads to increased oxygen consumption and oxidation of NADH. Hence, there is a large increase in the rate of O2 consumption when 2,4 dinitrophenol is added.
B) High concentrations of ADP are not inhibited by DNP. Instead they can be inhibited by AMP. DNP cannot be added instead of ADP because it acts as an uncoupler while ADP acts as a carrier. Without ADP no protons will be able to pass through the membrane and the experiment won't take place.
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