Gel #1 : Analyze the gel as a whole using the first gel picture on the next page
ID: 37711 • Letter: G
Question
Gel #1: Analyze the gel as a whole using the first gel picture on the next page. Complete the first column in the table below.
Gel #2, Student #4: Analyze the gel as a whole using the second gel picture on the next page. Analyze the quadrant of the gel for the designated student number. Complete the remaining columns in the table below.
Answer the following questions about the labeled gel. For the Explanation section, put an ?X? in boxes where the option is possible. Leave boxes where the option is not possible blank.
Gel number
1
2
2
2
Sample name
A-COI
A-COI
P-rbcL
B-16S
Number of PCR bands in the lane
Approximate sizes of the bands in the lane (bp)
Explanation
Ethidium bromide was not added to the gel
Component of the Taq mix was degraded
No primers in the master mix
DNA template not added to PCR tube
Not enough DNA in genomic DNA prep or
bacterial culture
Contamination of genomic DNA or PCR sample
Non-specific primer binding
Samples switched when loaded on the gel
PCR was performed correctly
Sample should be sequenced
Please Help!
Gel number
1
2
2
2
Sample name
A-COI
A-COI
P-rbcL
B-16S
Number of PCR bands in the lane
Approximate sizes of the bands in the lane (bp)
Explanation
Ethidium bromide was not added to the gel
Component of the Taq mix was degraded
No primers in the master mix
DNA template not added to PCR tube
Not enough DNA in genomic DNA prep or
bacterial culture
Contamination of genomic DNA or PCR sample
Non-specific primer binding
Samples switched when loaded on the gel
PCR was performed correctly
Sample should be sequenced
Gel #1: Analyze the gel as a whole using the first gel picture on the next page. Complete the first column in the table below. Gel #2, Student #4: Analyze the gel as a whole using the second gel picture on the next page. Analyze the quadrant of the gel for the designated student number. Complete the remaining columns in the table below. Answer the following questions about the labeled gel. For the Explanation section, put an a??Xa?? in boxes where the option is possible. Leave boxes where the option is not possible blank.Explanation / Answer
Gel # 1
Gel number
1
2
2
2
Sample name
A-COI
A-COI
P-rbcL
B-16S
Number of PCR bands in the lane
0
0
1
1
Approx Size of band (bp)
0
0
600
1500
Gel # 2
Gel number
1
2
2
2
Sample name
A-COI
A-COI
P-rbcL
B-16S
Number of PCR bands in the lane
1
2
1
1
Approx Size of band (bp)
700
700, 300
700
1500
Gel number
1
2
2
2
Sample name
A-COI
A-COI
P-rbcL
B-16S
Number of PCR bands in the lane
0
0
1
1
Approx Size of band (bp)
0
0
600
1500
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