1. Fill in the question blank (left column) with the best answer (right column).

Question

1. Fill in the question blank (left column) with the best answer (right column). There is only one best answer for each question/blank. Each question/blank is worth 3 points. Answer (right column) can be used more than once or not at all. A. None of the below B. Ratio of enzyme activity per total mg of protein C. Principally measures peptide bonds, Tyr, Trp, and polar amino acids D. Bestows equal charge to mass ratio E. Principally measures Tyr, Trp, and Phe while nucleic acids interfere with measurement F. Used to determine protein purity and molecular weight G. Ratio of enzyme amount per total volume H. Used as a comparison to determine an unknown I. Only disrupts hydrogen bonds J. Only disrupts di -sulfide bridges K. Used to determine efficiency of transfer L. Measures total protein amount

Explanation / Answer

L- bradford assay

B-specific acivity

F-comassie

J- disrupt disulphide bonds

I- disrupt Hydrogen bond

K- efficiency of transfer

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