1) An E. coli culture is growing exponentially at 37 o C in minimal-glycerol med

Question

1) An E. coli culture is growing exponentially at 37oC in minimal-glycerol medium with a generation (doubling) time of 120 min.

            a) If the birth length (LB) of cells in a culture growing in an enriched medium is 1.5

times greater than the LB of the cells growing in the minimal-glycerol medium, what is the generation time (g) of the cells in the enriched medium?

            b) What will be the ratios of the birth volume (VB) of the cells in the minimal-glycerol

and enriched media?

            c) What will be the time interval between the completion of one round of chromosome

replication and the beginning of the next round of chromosome replication in one of these cells?

            d) What would be the response of cells growing in the enriched medium to a 5-min

exposure to a sub-lethal but high dose of UV irradiation? (Explain the underlying molecular mechanism of the SOS as well as the effect on cell growth patterns.)

Explanation / Answer

1) Birth length of culture growing on enriched medium = 1.5 times Birth length of culture growing on minimal medium

Birth length of culture growing on enriched medium = 1.5

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.