2. (8 points) Predict whether the GAL gens ( Gal1, Gal7 Gal10 and Gal2 ) will be
ID: 40351 • Letter: 2
Question
2. (8 points) Predict whether the GAL gens (Gal1, Gal7 Gal10 and Gal2) will be produced in the following yeast strains under the conditions noted. Use + to indicate that the GAL enzymes are synthesized at greater than basal levels, and 0 to indicate that the enzymes are not synthesized.
Galactose absent
Galactose present
(a) wild-type
(b) Gal4-/Gal4-
(c) Gal3-/Gal3-
(d) Gal80-/Gal80-
Galactose absent
Galactose present
(a) wild-type
(b) Gal4-/Gal4-
(c) Gal3-/Gal3-
(d) Gal80-/Gal80-
Explanation / Answer
in wild type enzymes will be produced in the presence of galacose to metabolise it
Gal4 is termed as activator so in the presence of galactose enymes would be produced.
Gal 3 in inducer in will induce the synthesis of enzymes in the presence of galactose
Gal80 is the repressor inhbits the transcription by binding to the transcription activating domain of Gal 4
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