A research had two identical plates of Hela cells. The researcher performed a tr

Question

A research had two identical plates of Hela cells. The researcher performed a trypan blue viability test on the first plate. The second plate was transfected with a GFP expression plasmid. Six hours after the transfection the researcher observed the number of fluorescent cells and then performed a trypan blue viability test. The results are show below:

````````````````````````````````````````````````Plate 1``````````````````Plate 2

Trypan Blue Test`````````Blue````````` 50 ``````````````````````100

`````````````````````````````````Clear````````` 200``````````````````````150

Fluorescence Test```````Green ````````---```````````````````````` 75       

``````````````````````````````````Clear````````` ---````````````````````````175

Both qualitatively and quantitatively, what can you conclude about the actual procedure of transfecting the cells?

Explanation / Answer

Fluorescence Test is the actual test procedure for the transfection.

Trypan blue is to determine whether cells is viable or nonviabe.a viable cell will have a clear cytoplasm whereas a nonviable cell will have a blue cytoplasm is observed with trypan blue.

Green Fluorescent Protein (GFP):

An inherently fluorescent protein often found in marine organisms, such as jellyfish.

Since the discovery of GFP, several other fluorescent reporters have been introduced; e.g. yellow fluorescent protein (YFP).

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