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Below are described the phenotypes of 4 different mutant strains of E. Coli. Eac

ID: 40730 • Letter: B

Question

Below are described the phenotypes of 4 different mutant strains of E. Coli. Each strain carries only one mutation, what could the genotype of each strain be?

Strain 1: Grows on minimal medium with lactose as a sole source of carbon; mkaes blue colonies on minimal medium sucrose X-gal.

Strain 2: Does not grow on minimal medium with lactose as a sole source of carbon; makes white colonies on minimal medium sucrose X-gal IPTG; an antibody that specifically recognizes the B-gal is able to detect a protein in this strain when grown on minimal medium sucrose IPTG X-gal; an antibody that specifically recognizes the lactose permease is able to detect a protein in this strain when grown on minimal medium sucrose IPTG X-gal.

Strain 3: Makes white colonies on minimal medium sucrose X-gal IPTG; makes white colonies on minimal medium sucrose X-gal; the antibody specific for the B-Gal never detects anything.

Strain 4: Makes small colonies on minimal medium lactose; makes colonies that are of lighter blue than usual on minimal medium sucrose IPTG X-gal and on minimal medium glucose IPTG X-gal.

Explanation / Answer

Answer:

Strain 1:vibrio vulnificus.

Strain 2: Salmonella typhimurium LT-2.

Strain 3: Adenovirus.

Strain 4: M15 strain of E. coli.

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