In lane \"A\", how many bands appear on the agarose gel at the end of the experi

Question

In lane "A", how many bands appear on the agarose gel at the end of the experiment and the DNA has migrated through the gel? (You may need to reference the sheet that I handed out in class or reprint it out from MOODLE). v-HindIII Cut site -Dde 'cut site . Rsa cut site . Taqicut site o kb 11 kb 2. How would your answer differ if the genome of Bacillus termagualis (total size is 11 kb) was CIRCULAR? Indicate your answers below as you did for the linear genome above. . Thisis for the inear genomeRFLP dige t- 1 i- · AB C DE F G H I J K L M N O

Explanation / Answer

The linear segment shown has 11 segments of 1 kb each. In band A, we need to find out the number of fragments generated by Rsa enzyme as shown on the top of the well.

The number of fragments and their sizes are for lane A:

For lane B:

For lane C:

For lane D:

for lane E:

For lane F:

For lane G:

For lane H:

For circular genome:

lane A:

Lane B:

Lane C:

Lane D:

Lane E:

Lane F:

Lane G:

Lane H:

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