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as part of characterizing a newly discovered human gene, youare fortunate to hve

ID: 4182 • Letter: A

Question

as part of characterizing a newly discovered human gene, youare fortunate to hve available a full-length cDNA in a plasmidvector. If you were to sequence this cDNA, which parts of the genewould you expect to see represented in the cDNA sequence? A. the poly(A) addition signal and the UTRs B. the promoter proximal elements and the start and stopcodons C. all of the core promoter and an enhancer D. the branch point consensus sequences and the codingsequence E. the template for the poly (A) tail and the shine-dalgarnosequence Can you please really explain why its that answer, thanx sooomuch as part of characterizing a newly discovered human gene, youare fortunate to hve available a full-length cDNA in a plasmidvector. If you were to sequence this cDNA, which parts of the genewould you expect to see represented in the cDNA sequence? A. the poly(A) addition signal and the UTRs B. the promoter proximal elements and the start and stopcodons C. all of the core promoter and an enhancer D. the branch point consensus sequences and the codingsequence E. the template for the poly (A) tail and the shine-dalgarnosequence Can you please really explain why its that answer, thanx sooomuch

Explanation / Answer

      A. the poly(A) addition signal and theUTRs            Whenthe full length of the cDNA in a plasmid vector is to be sequenced,the part of the gene we would to see that             isrepresented in the cDNA sequence is the poly(A) addition signal andthe UTRs which means untranslated region.             Thisis because, in the synthesis of cDNA we first add a poly(A) tail to the 3'end of theDNA from where the             complementaryDNA is synthesized.             Asit is the DNA the segment of it is not translated and it has to betranslated, so, that segment is untranslated in the             cDNA.

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