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Chi Square Analysis question (Genetics) To assess Mendel\'s law of segregation u

ID: 41891 • Letter: C

Question

Chi Square Analysis question (Genetics)

To assess Mendel's law of segregation using tomatoes, a true-breeding tall variety (SS) is crossed with a true-breeding short variety (ss). The heterozygous tall (Ss) were crossed to produce the two sets of F2 data as follows: Using chi-square analysis, analyze the results for both data sets. Calculate X^2 values, and estimate the p values in both cases. From the analysis in part (a), what can you conclude about the importance of generating large data sets in experimental settings?

Explanation / Answer

a.

Phenotype

O

E

(O-E)

(O-E)2

(O-E)2/E

Tall

30

26.25

3.75

14.0625

0.536

Short

5

8.75

-3.75

14.0625

1.607

2.14

P = between 2 and 0.05. The null hypothesis is accepted as the difference between the expected and observed values is less.

Phenotype

O

E

(O-E)

(O-E)2

(O-E)2/E

Tall

300

262.5

37.5

1406.25

5.35

Short

50

87.5

-37.5

1406.25

16.07

21.42

P = less than 0.001. Therefore, null hypothesis is rejected since there is a significant difference between the observed and expected.

b.

As sample size increases, it leads to different results and provides more confidence on the values obtained.

Phenotype

O

E

(O-E)

(O-E)2

(O-E)2/E

Tall

30

26.25

3.75

14.0625

0.536

Short

5

8.75

-3.75

14.0625

1.607

2.14

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