Genotype Scenarios Possible Phenotypes 1. pACYC treated with ECORI and transform

Question

Genotype Scenarios

Possible Phenotypes

1.

pACYC treated with ECORI and transformed into E.coli.

Cells grown in LB agar with Tetracycline. Will there be Growth / No growth? (circle the right answer)

Bacteria sensitive to ___________________________________

Bacteria resistant to ___________________________________

2.

pACYC treated with EcoRV and transformed into E.coli.

Cells grown in LB agar with Chloramphenicol and Tetracycline. Will there be Growth / No growth? (circle the right answer)

Bacteria sensitive to ________________________________

Bacteria resistant to _________________________________

3

pACYC not treated with EcoRV or EcoRI and transformed into E.coli.

Cells grown in LB agar with Chloramphenicol. Will there be Growth / No growth? (circle the right answer)

Bacteria sensitive to _____________ ___________________ Bacteria resistant to ___________________________________

Genotype Scenarios

Possible Phenotypes

1.

pACYC treated with ECORI and transformed into E.coli.

Cells grown in LB agar with Tetracycline. Will there be Growth / No growth? (circle the right answer)

Bacteria sensitive to ___________________________________

Bacteria resistant to ___________________________________

2.

pACYC treated with EcoRV and transformed into E.coli.

Cells grown in LB agar with Chloramphenicol and Tetracycline. Will there be Growth / No growth? (circle the right answer)

Bacteria sensitive to ________________________________

Bacteria resistant to _________________________________

3

pACYC not treated with EcoRV or EcoRI and transformed into E.coli.

Cells grown in LB agar with Chloramphenicol. Will there be Growth / No growth? (circle the right answer)

Bacteria sensitive to _____________ ___________________ Bacteria resistant to ___________________________________

Explanation / Answer

1. Growth will be there

2. No growth

3. Growth will be there

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