Primase adds RNA primers during DNA replication, typically 7-10 nucleotides in l

Question

Primase adds RNA primers during DNA replication, typically 7-10 nucleotides in length. Why is an RNA primer required for DNA replication?

Primases lack proofreading abilities. Does this impact the fidelity of DNA replication? Explain your answer.

Primases, RNA polymerases, and DNA polymerases all complete chain elongation in the 5' to 3' direction. What would the consequences be if polymerases instead worked in the 3' to 5' direction?

Please answer in exact, lengthly detail and if you are not 100% sure, do not answer.

Explanation / Answer

Why is an RNA primer required for DNA replication?

answer ; RNA  primer is required to add the nuclotides to the DNA molecule with the help of primase enzyme , as the DNA polymerase cannot add the nuclotides to the directly to the template strand . primase has the ability to place the nuclotides in the opposite side of the strand ie., 5 prime direction ,so that the DNA polymerase can actually will elongate the chain by adding the nuclotides to the RNA primer

Primases lack proofreading abilities. Does this impact the fidelity of DNA replication?

answer  ; yes it has greater impact on the fidelity of the DNA replication , the complementary base pairing depends on the Primase activity which invoves the 3-5 prime exonuclease activity , as it adds the RNA primer intially ,it checks the misparing of nuclotides

Primases, RNA polymerases, and DNA polymerases all complete chain elongation in the 5' to 3' direction. What would the consequences be if polymerases instead worked in the 3' to 5' direction

directionality is more important in maintaing the fidelity of the enzyme    it cannot add nuclotides in the opposite direction as it is energitically unfvouarbale reaction of adding the nuclotides . the DNA POLYMERASE CAN ADD NUCLOTIDES IN THE 3 PRIME OH GROUP TO FROM PHOSPHO DIESTER BOND . THE DNA STRAND 5 PRIME SIDE OF DNA ENDS WITH PHOSPHATE AND 3 PRIME SIDE ENDS WITH OH GROUP , SO PLOYMERASE CAN ADD NUCLOTIDE TO THE 3 PRIME OH GROUP , SO THE DIRECTINALITY SHOULD ALWAYS BE IN 5 PRIME TO 3 PRIME DIRECTION . if the polymerase has to ad nuclotide in 5 prime to 3 prime direcion it cannot add the nuclotide becaus of the phosphate group

DNA starnd   DIRECTION   - 5 PRIME TO 3 PRIME

PLOYMERASE ACTIVITY -   5 PRIME TO 3 PRIME , AS 3 PRIME END HAD OH GROUP OF SUGAR , IT CAN ADD NUCLOTIDE

DNA STRAND 2 ; 3 PRIME TO 5 PRIME

POLYMERASE ACTIVITY - 5 PRIME TO 3 PRIME

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