QUESTIONS 29-31. To map the location of important E. coli operons, you do an int

Question

QUESTIONS 29-31. To map the location of important E. coli operons, you do an interrupted Hfr mating experiment. You take a wild-type Hfr E. coli strain and mate it with a F strain that is resistant to tetracycline and is Gal- Thr- and Trp-. You plate the bacterial mixtures onto medium that contains tetracycline and is either lacking threonine, containing 50 galactose, or lacking tryptophan. With your results, you produce the figure to the right. 40 29. Can Hfr strains mate with F strains? 30 A)Yes B)No 20 30. What was the relative order of genes on the bacterial lo chromosome? 31. What type of DNA transfer mechanism is involved in this experiment? A) General transduction B) Specialized transduction C) Transformation D) Conjugation E) Chemotaxis

Explanation / Answer

29. no they cannot mate.

30. a. threo--gal--try

31.d conjugation

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