PLEASE note that there may be (most likely be) more than one number that satisfi

Question

PLEASE note that there may be (most likely be) more than one number that satisfies the answer. For example, for the first question, #1 may not be the only answer.

PLEASE LIST ALL NUMBER THAT CORRESPONDS

ISP+OCZ+Y+

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

To enter your answer, list only the numbers of the situations that would produce the enzyme at significant levels.

I-dP+O+Z+Y+

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

crp- I-dP+O+Z+Y+

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

I-P+OCZ+Y-/I+P+O+Z-Y+

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

I+P-OCZ+Y-/I+P+O+Z-Y+

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

I-P+OCZ-Y+/I+P+O+Z+Y-

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

I+P+O+Z-Y+/I-P+O+Z+Y-

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

I+P+OCZ+Y+/ISP+O+Z-Y-

For this genotype, indicate whether the enzyme is indicated is produced at significant levels.

1 ?-galactosidase, no lactose present

2 ?-galactosidase, lactose present as sole carbon source

3 permease, no lactose present

4 permease, lactose present as sole carbon source

Explain your answer for Rating.

Explanation / Answer

1. since supersupressor is present.

no galactosidase or permease will be made irrespective of lactose presence

2. can not understand genotype properly to help you

3. can not understand genotype properly to help you

4. since Operator is constitutive, and cis acting, only galactosidase will always be made irrespective of lactose presence. but permease (Y-) will not be made by this merodiploid in absence of lactose. because I+ is present on other strand with O+ which can be repressed by the repressor in absence of lactose. Thus, 1,2,4 correct

5. 2. only permease will be made in presence of lactose as constitutive operator is under negative promoter where no gene will be transcribed. On other strand galactosidase is negative so will not be made. inhibitor is + so allolactose will prevent binding to operator and permease will be made.

6. permease will always be made irrespective of lactose presence as it is under cis acting constitutive operator while galactosidase will be made only when lactose is present.

7. both made in presence of lactose

8. trans acting superrepressor is present thus both will not be made irrespective of lactose presence.

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