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Which of the following statements is NOT true? A.PCR is a method to generate mil

ID: 42977 • Letter: W

Question

Which of the following statements is NOT true?

A.PCR is a method to generate millions of copies of DNA that you are interested in.

B.PCR cycles through temperatures that allow for denaturation, annealing and extension.

C.PCR is a method of DNA typing.

D.PCR requires the use of primers.

A.PCR is a method to generate millions of copies of DNA that you are interested in.

B.PCR cycles through temperatures that allow for denaturation, annealing and extension.

C.PCR is a method of DNA typing.

D.PCR requires the use of primers.

Explanation / Answer

PCR (polymerase chain reaction)(developed by Karry Mullis) is method where we generate million of copies of DNA through a various cycle and a cycle have 3 phase which have different temperature. these phase are denatuation, annealing and extension. the PCR needs primer, DNA template, a heat labile polymerase Taq polymerase, bivalent cation buffer solution and a monovalent cation. this whole process occur in Thermocycler.

so the ans is C - PCR is method of DNA typing

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