For example: The non nucleotide prooducts obtained when oxaloacetate is added to

Question

For example: The non nucleotide prooducts obtained when oxaloacetate is added to a D20 solution containing malate dehydrogenase and NADH

The answer is:

CO2-

DO- H

CO2-

SOOOOOOOOO how would you do these:

a) The non nucleotide prooducts obtained when L malate is added to a D20solution containing malic enzyme and NADP+............

I know the answer is Pyruvate + CO2 ( where does the D2O come in)

and

b) The major product (s) formed when fructise 1, 6 bisphosphate is incubated with aldolase in D2O

The answer is dihydroxyacetone phosphate and glyceraldehyde 3 phosphate again...Where does the D2O come into place

Explanation / Answer

All lyophilized samples are dissolved in D2O and again lyophilized. So here D2O is uesd as a major material.

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