1) Does the graph of the data points show the Beer\'s Law is obeyed? 2) Does Abs

Question

1) Does the graph of the data points show the Beer's Law is obeyed?

2) Does Absorbance or Transmitance give a linear relationship to concentration?

Can someone please help me answer these question regarding Absorbance and Transmitance? Thank you.

3) Consider the color of DCIP an lack of color of DNA, do these spectra seem reasonable?

Absorbance and Tranmitance Spectra of 3x10A-5 DCIP 0.7 T 100 90 0.6 80 0.5 70 60 ai E 0.4 50 3 0.3 40 30 0.2 20 0.1 10 350 400 450 500 550 600 650 700 750 Wavelenth (nm) Absorbance Transmitance

Explanation / Answer

1) The graph show the realtion between absorbance and wavelength.

The Beer law gives relation between absrobance and concentration

A = e.c.l ........(1)

so we obtain a straight line if we plot a graph between absrobance and concentration.

2) Yes the relation between absrobance and concentration is linear , as shown above in the equation 1

3) the colour spectrum is observed in Visible region of spectrum

400-800nm is the range of visible region.

So there is no colour in the given solution as the maximum absorbance is near 260 nm .

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.