View/perform/read ALL THREE of the following prior to answering the questions. h

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View/perform/read ALL THREE of the following prior to answering the questions.

http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro10.swf::Steps in Cloning a Gene (Links to an external site.)

http://www.discoverbiotech.com/wiki/-/wiki/Main/Applications of Cloning (Links to an external site.)

http://www.wiley.com/college/boyer/0470003790/animations/cloning/cloning.htm (Links to an external site.)

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From the list below, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I.              Transform bacteria with recombinant DNA molecule
II.           Cut the plasmid DNA using restriction enzymes
III.         Extract plasmid DNA from bacterial cells
IV.          Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments
V.            Use ligase to seal plasmid DNA to nonplasmid DNA

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Plasmids (or vectors) are important in biotechnology because they are

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Plasmids are put into bacterial cells by

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Restriction enzymes usually

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After combining DNA fragments in a cloning experiment, ___ is used to covalently join the DNA segments.

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It is theoretically possible for a gene from any organism to function in any other organism. Why is this possible?

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Assume that you are trying to insert a gene into a plasmid and someone gives you a DNA sample cut with restriction enzyme X. The gene you wish to insert from the given sample has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to

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Which of the following is/are false in regard to expression plasmids (also called expression vectors)?

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What is NOT a potential problem(s) associated with using bacteria containing a cloned eukaryotic gene (e.g. a human gene) to produce a functional protein?

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Cloning allows for production of proteins in much larger amounts than occurs in the cells from which the gene is isolated.

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Question 111 pts

Gene cloning is used to do all of the following except

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In your

Explanation / Answer

Ans.1) The correct sequence of insertion of foreign DNA into a plasmid and inserting the plasmid into a bacterium -- III, II, IV, V, I

2.) Plasmids (or vectors) are important in biotechnology because they are -- a vehicle for the insertion of recombinant DNA into bacteria.

3.) Plasmids are put into bacterial cells by -- transformation.

4.) Restriction enzymes usually -- make staggered cuts at specific sequences in DNA in both donor DNA and plasmid.

5.) After combining DNA fragments in a cloning experiment -- DNA ligase -- is used to covalently join the DNA segments.

6.) It is theoretically possible for a gene from any organism to function in any other organism. Why is this possible -- All organisms have the same genetic code.

7.) Cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.

8.) Which of the following is/are false in regard to expression plasmids (also called expression vectors) -- They are the first plasmid type used to clone a gene- This statement is false. The first plasmid type used to clone a gene is pBR322.

10.) Cloning allows for production of proteins in much larger amounts than occurs in the cells from which the gene is isolated -- True

11.) Gene cloning is used to do all of the following except -- perform gene therapy.

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