for this lab we pipette 50uL from an original 100% food color concentration in w

Question

for this lab we pipette 50uL from an original 100% food color concentration in well A1. We transfer 50uL from A1 to B1, mix and then transfer 50uL from B1 to C1 mix and so on until we reach well G1. What is the concentration of the color for each well as it is passed from well to well.

Thanks

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Explanation / Answer

Let Cinitial = 100%

C1*V1 = C2*V2

C2 = C1*V1/V2 = 100*50/(50+50)

C2 = 50% of original

Then

C2*V2 = C3*V3

C3 = C2*V2/V3 = 50*(100)/(100+50)

C3 = 33.33% of original

finally

C3*V3 = C4*V4

C4 = C3*V3/V4

C4 = 33.33*(150)/(150+20) = 29.4% from original

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