The activity of an enzyme known as polyphenoloxidase is studied. Through the act

Question

The activity of an enzyme known as polyphenoloxidase is studied. Through the action of this enzyme, the substrate known as catechol is converted to o-quinone. Various concentrations of substrate are shown in the table blow as are V_0 values for each concentration. In addition, data for two inhibitors (PHBA and PTU) is also shown. From these data, determine the K_m without inhibitor. From these data, determine the V_max without inhibitor. What type of inhibitor is PHBA? What type of inhibitor is PTU?

Explanation / Answer

For the given enzymatic reaction,

a. Km for without inhibitor = 0.45 mM

b. Vmax for without inhibitor = 0.081 umol/min

c. the Km and Vmax for reaction with PHBA are lower than without inhibitor, so the type of inhibition for PHBA is uncompetitive inhibitor.

d. the Km and Vmax for reaction with PTU are lower than without inhibitor, so the type of inhibition for PTU is uncompetitive inhibitor.

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