You have 3 ml of a 300 mg/dl glucose solution and you need to make 3ml of a 150

Question

You have 3 ml of a 300 mg/dl glucose solution and you need to make 3ml of a 150 mg/dl solution how do you make this dilution? Make a 75 mg/dl solution using a 450 mg/dl stock solution. How many mu l (microliters) of serum will be needed to make 500 mu l of a 1 to 5 dilution in saline? A sample of 200 mu l of blood needs to be diluted 1 to 50. What will be the final volume of this dilution? You need to make 50ml of a 1/250 dilution of urine in distilled water. How much diluent will you need? Explain the difference between dilution and ratio If a 500 mu l of a solution contains 450 mu l of diluent, what is the dilution and ratio for this solution? How would you prepare 1 ml of a 2/5 dilution in saline? An instrument measuring LDH has a linearity limit of 1500 IU/L, your patient s LDH is over 11, 250 IU/l what smallest dilution would you need to bring the patient value into measurable range? Dilution using 1 part serum to 3 parts saline would be stated as a _____ dilution?

Explanation / Answer

1) Concentration of glucose = 300mg/dl

1 ml= 0.01dl, So 3 ml = 0.03dl. So weight of Glucose (w) = 300mg/dl * (0.03dl) = 9mg

Now we need 150mg/dl solution. Let V be the final Volume of the solution.

So w/V = 150mg/dl

V = w/150 dl = 9/150 dl = 0.06dl or 6ml

So we need to add more 3ml of water to the initial solution of 3ml of 300mg/dl Glucose solution.

2) Initial Concentration = 450mg/dl

We have to dilute it to 75 mg/dl that is reduce the concentration by 6 times. So we need to add 5ml of water to the 1ml of 450mg/dl solution of glucose.

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