Calibration Curve Y 1.09X 0.02 Equation of the Best-Fit Line What quantity does

Question

Calibration Curve Y 1.09X 0.02 Equation of the Best-Fit Line What quantity does Y represent? What is the unitof Y? What quantity does X represent? What is the unit of Samples Corrected Concentration (/ml) 0.560 mixture (trial 1) dilution factor mixture (trial 2) 0.570 dilution factor mixture (trial 30 dilution factor Average protein concentration in the mixture SUAam 0.470 GPC pure (trial 1) GPC pure (trial 10 0.490 GPC pure (trial 1) 0.500 Average protein concentration for GPC-purified protein in peak one GPC pure (trial 1) 0.220 Peak 2 pure (trial 2) 0.250 Peak 2 0.210 GPC pure (trial 3) Peak 2 Average protein concentration for GPC purified protein in peaktwo b Assume you ran 1 ml of mixture to purify on your Sephadex G-100 (GPC). You

Explanation / Answer

Callibration curve is the graph of concentration verses singnals obatin from the instrument.

For example uning UV-Visible spectrophotometer one can record the aborbance for different concentration samples.

Thus graph of concentration verses those obatin absorbance for different samples is the callibration curve.

From the Beer law we know that the absorbance of the sample is directly proportion to its concentration.

After ploting the graph the best fit line equation is obtain.

In above eg. we have equation: Y= 1.09X + 0.02

where Y= Absorbance for above example or it may be the value of instrument responce.

Depending on the graph ploted the unit of Y may change, but in above example Y= aborbance which is unitless.

X represents the concentration.

Unit of X is mol per liter (molL-1)

Related Questions

Navigate

• Browse (All)
• Browse C
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.