For each of the following circle all correct answers unless instructed otherwise

Question

For each of the following circle all correct answers unless instructed otherwise in the question. Which of the following would have the greatest buffering capacity at pH = 6? 0.050 M carbonic acid/bicarbonate system, pk 6.4 0.100 M carbonic acid/bicarbonate system, pk 6.4 0.250 M acetic acid/acetate system, pk 4.76 0.100 M acetic acid/acetate system, pk 4.76 0.100 M histidine (see pK's shown above) 0.010 M histidine (see pK's shown above) Which of the following statements apply to dialysis? Choose all correct answers. Can be used medially in artificial kidney machines to remove urea Involves the movement of small particles across a semipermeable membrane Involves the movement of water across a cell membrane may be used to remove small molecules and ions from a protein solution.

Explanation / Answer

Q1.

buffering capacity = how much they will withstand pH change as ACID or BASE is added...

Note that histidine ha up to three pKa's values:

1.82; 9.17; and, most importantly, its carbn chain --> 6.00

So we have 2 choices either 0.1 M of 0.01

So

The total addition of histidine will be better, since there is higher concentrations, so choose E

0.1 M of histidine with pK values of 6.4

Q2

a) is correct, since it is used to get rid of acidic urea in blood

b) Also true, it works as a filter from particles

c) Also true, since water flows, we must "clean" water

d) False, it wont be used for protein solutions, mainly ions in blood/water

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