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QUESTION 4 After digesting the PCR product for the normal A allele with Mstll, t

ID: 48229 • Letter: Q

Question

QUESTION 4 After digesting the PCR product for the normal A allele with Mstll, the following sizes of band(s) is/are observed on gel electrophoresis a. 200 bp only b. 300 bp only c.500 bp only d. 200 bp and 300 bp e. 200 bp, 300 bp, and 500 bp QUESTION 5 After digesting the PCR product for the sickle S allele with Mstll, the following sizes of band(s) is/are observed on gel electrophoresis:_. a. 200 bp only Ob. 300 bp only c.500 bp only d. 200 bp and 300 bp e. 200 bp, 300 bp, and 500 bp QUESTION 6 Genotyping a person with genotype AS using the methods described above would result in the following sizes of band(s) on gel electrophoresis a. 200 bp only Ob. 300 bp only c. 500 bp only d. 200 bp and 300 bp e. 200 bp, 300 bp, and 500 bp

Explanation / Answer

4.

The size of band observed will be (e) 200 bp , 300 bp and 500 bp

5.

The size of band observed will be (c) 500 bp only

6.

THe size of band observed will be (d) 200 bp and 300 bp

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