3) How many pieces of DNA would result from digesting human genomic DNA with the

Question

3) How many pieces of DNA would result from digesting human genomic DNA with the restriction enzyme PacI? PacI cuts the DNA sequence TTAATTAA. Note only one strand of DNA is shown here. The human haploid genome contains 3x10^9 bp of DNA. For this question, assume that A, C, G, T are in equal frequencies.

a) 512
b) 1024
c) 4,096
d) 45,776
e) 65.536

4) What charge does DNA have, and what gives it this charge?
a) positive, from the phosphates
b) positive, from the bases
c) negative from the phosphates
d) negative, from the bases
e) negative, from the deoxyribose

6) At a particular locus on the X chromosome, John and Jim have the DNA sequences indicated below:
John: AGCTTACGAAACC
Jim: AGATTACGAAACC
The difference in the two sequences represents an:
a) indel
b) SNP
c) microsatellite
d) minisatellite

Explanation / Answer

4. DNA has negative charge , from phosphates.

6. SNP . They differ in one nucleotide .

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