(I am confused as to how to interpret this graph so that I can properly answer t
ID: 482919 • Letter: #
Question
(I am confused as to how to interpret this graph so that I can properly answer the question.) An answer with detailed response is appreciated)
Acrobat Pro File Edit View Window Help Mon 1:42 PM a O E OLD (FL14) BIO406 EXAM No Answers pdf Home Tools Assign. Protein OLD (F x Intro Bi... Amino... Familia. Tay-sa... Kwashi... Hutchi... Sign In 108% Find your tools here For questions 22 and 23, refer to the salt-precipitation plot for isolating proteins (below). Create PDF Edit PDF Export PDF henngl globulin pse Comment myoglobin Serum Organize Pages albumin C E -0.5 fibrin Enhance scans Protect 1.0 Fill & Sign 10.0 2.0 Prepare Form lonic Strength nr NH4)2s0. Snlu Linn (M) 4 Day(s) Left in Trial 22 If you were to slowly increase the amount of 23. If you were to slowly increase the amount of Buy now to continue using Acrobat NH4 4, W protein would you isolate first? hich NHAD2So4 which protein would you isolate last? without interruption. a. Hemoglobin a. Hemoglobin Buy Now ActivateExplanation / Answer
Looking at the graph shown above,
as the amount of (NH4)2SO4 is increased in the solution, the ionic strength of the solution also goes up.
The protein that would be isolated at high (NH4)2SO4 concentration would be, fibrinogen, which elutes at relatively lower ionic strength of (NH4)2SO4.
The protein myoglobin (elutes at the end) sticks strongly and does not gets eluted until the concentration of (NH4)2SO4 in solution is very high and all the other proteins had been eluted already.
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