The isoelectronic point of an amino acid is the point where the pK_a of the alph

Question

The isoelectronic point of an amino acid is the point where the pK_a of the alpha-carboxylic acid is the same as the pK_a of the alpha-amino group. In Gel Filtration Chromatography, a protein mixture must be applied that the proteins will have a net positive charge and bind to the column. In order for DEAE to act as an anion exchanger, it must have a positive charge. One of the reasons the primary structure is important for a protein is that it determines the three dimensional shape the molecule adopts in aqueous solutions In general, proteins are least soluble in water when the pH is far away from the pI. Spontaneous processes are characterized by a change in Gibbs free energy that is positive.

Explanation / Answer

Ans26- In gel Filtration chromatography protein is seprated by a set of proteins with the help of its size. Gel type chromatography is also known as size exclusice chromatography (SEC) we use buffer solution in this to give iso-electric point of stabilization.This technique is used for purification of sampleof high weight contaminants.The mass of an unknown protein can be estimated by its elution position. As we lower the pH the net positive charge in the colunm decreases hence the column is negatively charged so so hace stability or to give iso-electric stability the protine with the net charge will bind with the coloumn but this process in unlike ion exhange chromatogaphy but the mass of an unknown protein can be estimated by its elution position where the protine is stuck in the bead.

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