Your research project requires that you purify protein A away from proteins B an

Question

Your research project requires that you purify protein A away from proteins B and C using a combination of gel filtration chromatography and ion-exchange chromatography. Describe a purification scheme using these two columns that would accomplish your goal with the least amount of protein dilution Include in your answer the types of running buffers you would use ionic strength and pH) and the expected order of elution of the proteins from the two columns, given the physical properties in the following table:

Explanation / Answer

Separation of proteins

First we would do a gel filtration wherein, protein A and protein C would separate out of protein B.

Protein B with higher molar mass s separated.

Then using, ion-exchange chromatography we can separate Protein A and protein C as their pI are different.

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