biochemistry please provides explanations with all answers 2. (15 points) A nona

Question

biochemistry
please provides explanations with all answers

2. (15 points) A nonapeptide was determined to have he following amino acid composition: (Lys)2, (Gly)2, (Phe)2,His, Leu, Met. The native peptide was incubated with Sanger's reagent and then hydrolyzed; 2,4- dinitrophenyl methionine was identified by HPLC. Incubation of the native peptide with trypsin gave two tetrapeptides, and free His. ethionine was recovered from one of the tetra peptides, and 2,4-Dinitrophenyl- phenylalanine was recovered from the other tetrapeptide, Digestion of the native peptide with the enzyme pepsin produced two dipeptides, and a pentapeptide. The pentapeptide was composed of (Gly)2, Phe, Lys and His. Determine the native sequence: Show all work and fragments for each digestion/step for your choice Vhe. The s His His

Explanation / Answer

Digestion with the enzyme pepsin produced a dipeptide, a tripeptide, and a tetrapeptide.

The tetrapeptide was composed of (Lys)2, Phe, and Gly. The native sequence was determined to be:

. A nonapeptide was determined to have the following amino acid

       composition: (Lys)2, (Gly)2, (Phe)2, His, Leu, Met.

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2. The native peptide was incubated with 1-fluoro-2,4-dinitrobenzene (FDNB) and then hydrolyzed; 2,4-dinitrophenylhistidine was identified by HPLC.

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3. When the native peptide was exposed to cyanogen bromide (CNBr), an octapeptide and free glycine were recovered.

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4. Incubation of the native peptide with trypsin gave a pentapeptide, a tripeptide, and free Lys. 2,4-Dinitrophenyl-histidine was recovered from the pentapeptide, and 2,4-dinitrophenylphenylalanine was recovered from the tripeptide.

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5. Digestion with the enzyme pepsin produced a dipeptide, a tripeptide, and a tetrapeptide. The tetrapeptide was composed of (Lys)2, Phe, and Gly. The native sequence was determined to be:

answer

His-Leu-Phe-Gly-Lys-Lys-Phe-Met-Gly

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