Please provide me with an easy instructions to follow this lab expirnments, plea

Question

Please provide me with an easy instructions to follow this lab expirnments, please number the steps and explain to me op-6-22 please.
Thanks! 15 XPERIMENT Part II Laboratory Experiments Correlated Chromatographic Analysis of tha Qualitative Analysis Thin-Layer Chromatography. Operations Making Chromatography OP-22 Thin Before You Begin review oP-22 carefully, and men read or the plan experimental Scenario of drug tablets wa A patient who swallowed a large number suffering from a into the emergency room of a local health clinic some unused dose. The drug is pousc recalls were left on a counter in the bathroom. The patient's medicine analgesic drug in the a bottle of an on the label. Because doesn't remember what was there was no toxicology lab in operation, the clinic rushed the tablets to the for analysis. Your assignment is determine the identity of emergency room physicians to treatment. can apply the appropriate drugs sota Applying Scientific Methodology This experiment requires you to identify the components of an analgsi drug tablet and then identify the tablet as one of the ommercial dry preparations listed in Table 15.1. You will probably not be able to formuldi a meaningful hypothesis until after you examine your thi chromal raphy (TLC) plate under ultraviolet light. Then you can use another on method to provide evidence that may either support tentative hypothesis. Table 15.1 Composition of some analgesic drug prepara ions (in milligrams per tablet) Drug name Aspirin Acetaminophen Ibuprofen Salicylam Advil 200 Anacin Aspirin. 325 BC Tablets 325 Excedrin 250 *5-grain Lahlcu (1 grain 64.8 mg)

Explanation / Answer

Thin layer chromatographic analysis of drug component

Aim: Analysis of composition in some analgesic drug by thin layer chromatography

Chemicals and materials required: Dichloromethane, methanol. Ethylacetoacetate, acetic acid, paper wick, TLC chamber, stirring rod, Test tube, vials

Experimental steps

Preparation of the developing chamber

1. Prepare eluent that is solvent mixture for the chamber. Take ethylacetoacetate and acetic acid in 200:1 ratio

2. Fill the developing chamber with above eluent containing a paper wick to a depth of about 5 mm with the solvent.

3. Cover the developing chamber with plastic wrap or an appropriate lid and slosh up the sides of chamber to moisten the wick.

4. Keep developing chamber aside under a hood

Preparation and development of the TLC plate

1. Take one quarter of the unknown analgesic drug and grid to fine powder using flat-bottomed stirring rod.

2. Place the fine powdered drug in a test tube

3. Prepare 1:1 mixture dichloromethane and methanol

4. Add 2.5 mL of the above mixture to the finely powdered drug. Thoroughly mix the solid in the solvent with a stirring rod to dissolve as much of the solid is possible

5. Use a filter-tip pipet to transfer the solution to a small vial leaving the solid behind and cap the vial.

6. Take the silica gel plate which has fluorescent indicator. Spot the unknown solution and all of the standard solutions provided on the silica gel plate using different micropipet and label the spots.

7. Develop the plate under the hood in the developing chamber. Do not disturb the chamber.

8. Mark the solvent front before the plate dries.

Visualizationa and analysis

1. Place the dried TLC plate in the UV Chamber (short wavelength 254 nm).

2. Outline the spot with pencil and mark the greatest intensity.

3. Record the spots characteristics and compare with the standard.

4. Find out the Rf values. Identify the active ingredients of your unknown drug by comparison with the Rf values and visualization results for the known spots.

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.