How does chromatography work in the terms of intermolecular forces of attraction
ID: 494210 • Letter: H
Question
How does chromatography work in the terms of intermolecular forces of attraction, the interplay between the mobile phase, the paper coating and the dyestuff? Why are the dyestuffs separated to different degrees by different solvents in the mobile phase? What is the influence of the solid phase on the separation? What characteristics make a good chromatography solvent? How does chromatography work in the terms of intermolecular forces of attraction, the interplay between the mobile phase, the paper coating and the dyestuff? Why are the dyestuffs separated to different degrees by different solvents in the mobile phase? What is the influence of the solid phase on the separation? What characteristics make a good chromatography solvent? Why are the dyestuffs separated to different degrees by different solvents in the mobile phase? What is the influence of the solid phase on the separation? What characteristics make a good chromatography solvent?Explanation / Answer
In chromatography the main role is of the intermolecular forces of attraction as it depends on the forces of attraction with which a organic compound is adsorbed by the solvent . In chromatography paper is prepared by applying silica coating on the paper as silica is best adsorbent of almost all the organiv compounds. Mobile phase is the particular solution in whuch the chromatographic paper is dipped and allowed to run on the basis of adsorbance of the unknown organic compounds. Dye stuffs are the compounds which we have to separate by chromatography method.
Dye stuffs are separated to different degrees by different solvents in mobile phase due to the presence of different compound present in the dye stuff according to their respective adsorbing power and rf value.
A solid phase or a stationary phase has a very great influence om the separation as it holds the dye stuff according to their adsorbance value .
Characteristics that make a good chromatography solvwnt
. Good adsorbent
Solubility
should not dissolve the organic compound to make a new compound
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