a. Describe the process of mismatch repair and the alternative products. Say a r
ID: 49552 • Letter: A
Question
a. Describe the process of mismatch repair and the alternative products. Say a replication error changes an AT pair to AG. What are the two alternative products resulting from the repair of the AG, and outline in detail the steps of repair and how the alternative products arise.
b. Given the illustrated Saccharomyces cerevisiae tetratype in which reciprocal heteroduplexes have been generated by cross over and branch migration (see above). What are all the tetrads, and their ratios that result from the resolution of the heteroduplexes.
Strand 1 has A and A/a
Strand 2 has a and A/a
Explanation / Answer
answer for a ;
steps;
answer for B ;
the formation of heteroduplex is possible only with holliday model of recombination , where there will be cross bridegs ,branch migration . this is seen mainly during the mismatch repair followed by splicing the molecule
the ratio will be 4;4 , when there no correction
the ratio will be 5;3 , when only one heteroduplex is corrected
the ratio will be 6;2 ,when both heteroduplex is corrected
in the above case the there will be ration of 4;4 , because of the reciprocal heteroduplex ,resolution is same in both the strand
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.