1/Recombinant plasmids are engineered so that they can replicate in the cell ind
ID: 49664 • Letter: 1
Question
1/Recombinant plasmids are engineered so that they can replicate in the cell independently of the chromosome replication. Why is it important to have multiple copies of a recombinant plasmid within a cell?
2/How is the information encoded in the rfp gene expressed as a trait? Be sure to use what you have previously learned about gene expression and the relationship between DNA, RNA, protein, and traits.
3/Why is it possible for bacteria to make a human protein, such as insulin, or a sea anemone protein, such as the red fluorescent dye?
Explanation / Answer
1. Recombinant plasmids are that plasmid which carried DNA sequence of our need. we know plasmid are extra circular self replicating DNA molecule occur in the bacteria. when we need multiple copy of target DNA sequence or we want to know the gene expression of target DNA sequence so target DNA inserted into plasmid. these plasmid by the nature of self replicative forms multiple copy of recombinant plasmid these plasmid are same as parents plasmid. this phenomenon is called clonning.multiple recombinant plasmid form multiple copies of DNA thats why it is important to have multiple copies of a recombinant plasmid within a cell.
2. rfp gene occur in plasmid when we go for gene product i.e. protein we detect the trait of rfp gene if it is occur it means plasmid give. rfp gene is expressed into mRNA, translated into a protein, which fluoresces (the trait is red fluorescence).
3. we know that gene specific in their function. gene forms protein via mRNA and protein id encoded by genetic code and genetic code are universal so the gene will encode the same protein regardless of which organism is making. bacteria have only coding region while human have both coding as well as non coding However, it rarely works using a native eukaryotic gene for expression in bacteria, because the former have introns which the latter don't have the machinery to remove.
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