The prediction of protein coding regions in eukaryotic genomic sequences is made

Question

The prediction of protein coding regions in eukaryotic genomic sequences is made difficult by the presence of ______. Since exons can be small, it can be difficult to determine if an open reading frame is _____ or just a chance occurrence. When genomic sequence is being analyzed, the ____ is used as a limit to set what will be considered as an exon as opposed to what will be consdiered as non-coding sequence. The actual limit that is set to predict what might be an exon is frequently _____. Because of this limitation of analysis, the ____ for a protein may not be identified.

Explanation / Answer

1. The prediction of protein coding regions in eukaryotic genomic sequence is made difficult by presence of part of an intron.

2. SInce exons can be small, it can be difficlut to determine if an open reading frame is for translation of a protein or just a chance occurence.

3. When a genomic sequence is being analysed, the number of amino acids is used as a limit to set what will be considered as an exon as opposed to what will be considered as non-coding sequence.

4. The actual limit that is set to predict what might be an exon is frequently 21 amino acids.

5. Because of this limitation of analysis, the regulatory region for a protein may not be identified.

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