An interrupted conjugation experiment is conducted using the following strains:
ID: 49942 • Letter: A
Question
An interrupted conjugation experiment is conducted using the following strains: HfrStrRx+y+z+ and F- StrSx-y-z-. Samples of exconjugants are tested at regular intervals for their ability to grow on minimal medium (MM) containing streptomycin plus compound X, Y, or Z. The table below summarizes the number of colonies obtained on the different media when conjugation was interrupted after 10, 15, 20, or 25 minutes.
Media used
Time of interruption
MM+Str+
MM+Str+X
MM+Str+Y
MM+Str+Z
10 min
5
0
0
0
15 min
10
0
10
0
20 min
250
15
250
0
25 min
250
250
250
10
Based on these results, what is the order of entry of the four markers in question?
Possible answers:
Media used
Time of interruption
MM+Str+
MM+Str+X
MM+Str+Y
MM+Str+Z
10 min
5
0
0
0
15 min
10
0
10
0
20 min
250
15
250
0
25 min
250
250
250
10
Explanation / Answer
The correct answer is Str y x z
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