Question 2 The sequence of Drosophila 19-22 (without GST) is the following: NSAY

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Question 2 The sequence of Drosophila 19-22 (without GST) is the following: NSAYLeFM WKADVIESWI DDKENYVRSD EFGRDLSTve TLLTKeETFD AGLNAFEREG IHNI TALKDe LINASHAeSP AILKRHGDVI ARWORLRDAS NTRKDRLLAM eE FReIEEL YLTETAKKASA FNSWFENAEE DLTDPVRCNS IEEIRAALRD HA FOASLSS AEADFKALAA A LDOK IKSFNV GPNPYTHFTM EALEETHRNL eKIIEERDGE LAKEAKROEE KLRKEFAKT HANLE HOWLT ETRTSMMEGS GSLEROLEAL RVRKAATEVRAR RVDLKKIEEL GALLEEHLIL DNRYTERSTV GLA00WDOLD QLSMRMOHNL E00IQARNHS GVSEDsLKEFT SMMF KHFDKD KSGRINHOEF KSCLRAL LPMVEEGOPD PEFEAILDvv DPNRDGYVSL QEYIAFMISK GYD ETENVOSYEE IENAFRAITA ADRPYVTKEE LYCNLTKDMA DYC VORMKPF SEPRSGOPIK DALDY IDFTR TLFON In this sequence the repeating segments o19 and o21 are highlighted in yellow, while segments ou20 and 022 are not highlighted. The following table shows the molecular weight of the various polypeptides (in g/mole or daltons): Segment/protein Molecular Weight 19 13,336 13,145.5 020 021 12,609 021-LELY 1972.5 022 18218.5 GST 25,499 Lo-chymotrypsin 25.053 a) Calculate the concentrations (in moles per liter) of Fo 19-22 WT and chymotrypsin used in the reaction. 2oul of Fo19-22 2mg/ml) and 5 kul of chymo sin (a 0.1 mg/ml) were used in the reaction (final volume: 150 ul) b) Calculate the chymo Fou19-22WT molar ratio in the reaction mixture Show your calculation. c) Is the chymot ratio higher or smaller than the chymot Assume that the concentrations and volume of proteins are the same as above (part 1). Explain.

Explanation / Answer

The concentration of F22 is highest as compared to the other fragments or segments. Chymotrypsin is a protease, and it cleaves the carbonyl side of some peptide bonds by covalent catalysis. It catalyzes the reaction rate by a factor of 109. Alpha chymotrypsin is one of many crystalline enzymes and its properties can be investigated on molecular basis. It has a molecular weight 24,800. The concentration of this enzyme can be calculate using certain kinetic equations.

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