Shown in the following diagrams arc the absorption and fluorescence spectra and
ID: 501622 • Letter: S
Question
Shown in the following diagrams arc the absorption and fluorescence spectra and the structure of riboflavin. Refer to this diagram when answering the following questions. Please ignore the spelling error along the x-axis. It is not mine. a. Which spectrum (red or blue) is the absorption spectrum? Which spectrum is the fluorescence spectrum? Rationalize your choices. b. What is the best wavelength at which to excite riboflavin in order to observe its entire fluorescence spectrum? Why? c. What is the best wavelength at which to monitor the fluorescence of riboflavin-containing solutions? Why? d. Which part of the riboflavin molecule is responsible for its fluorescent properties? Explain.Explanation / Answer
Given the spectrum for Riboflavin
a. The blue line in the spectrum represents the absorption spectrum for Riboflavin. Ribovlavin absorbs at a lower wavelength in the UV spectrum. This is clearly shown from the graph above.
The blue line represents the fluorescence spectrum of Riboflavin. the molecule shows a a maxima at around 500 nm. the fluroresence shown by the molecule is very strong as the excitation occurs very easily.
b. The best excittion wavelength for Riboflavin while consulting the chart above, would be about 450 nm. At this wavelength the moelcule absorbs light and electrons easily gets excited, while coming back or relaxing to the ground state they release the absorbed ligth which is measured in the fluoresence spectrum.
c. The best wavelength is about 500 nm to monitor the fluoresence as the wavelength maxima is at that point so any small change in concentration of riboflavin in the solution would be seen in the intensity of spectrum.
d. The fully aromatic and conjugated part of Riboflavin is responsible for the fluoresence spectrum seen above. the easy excitation occurs due to extensive resonance in the molecule at room temperature.
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