8. An unretained solute passes through a chromatography column in 3.7 min and th

Question

8. An unretained solute passes through a chromatography column in 3.7 min and the analyte requires 8.45 min.
(a) Find the adjusted retention time and retention factor for the analyte. (b) The volume of the mobile phase is 1.4 times the volume of the stationary phase. Find the partition coefficient for the analyte.
8. An unretained solute passes through a chromatography column in 3.7 min and the analyte requires 8.45 min. (a) Find the adjusted retention time and retention factor for the analyte (b) The volume of the mobile phase is 1.4 times the volume of the stationary phase. Find the partition coefficient for the analyte.

Explanation / Answer

Adjusted retention time = 8.45 min- 3.7min = 4.75 min

retention factor = 4.75 min/3.7 min = 1.28

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Vm/Vs = 1.4

or, Vm = 1.4Vs

Partition coefficient = retention factor * (Vm/Vs)

                               = 1.28 * (1.4Vs/Vs) = 1.797 ~1.8

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