QUESTION 5 In experiment 2, what did the color change of bromothymol blue (from

Question

QUESTION 5

In experiment 2, what did the color change of bromothymol blue (from blue to green to yellow) indicate?

beans were carrying out photosynthesis

beans were carrying out fermentation

the solution was becoming more acidic

beans were producing oxygen

10 points   

QUESTION 6

What is the mechanism driving the color change in the bromothymol blue solution?

the oxygen produced from respiration combines with water to form hydrogen peroxide, which makes the solution acidic

the oxygen produced from respiration combines with water to form hydrogen peroxide, which makes the solution basic

the carbon dioxide produced from respiration combined with water to form carbonic acid, which makes the solution acidic

the carbond dioxide produced from respiration combined with water to form carbonic acid, which makes the solution basic

10 points   

  

QUESTION 9

The active sites of enzymes bind which of the following?

coenzymes

cofactors

substrates

regulators

10 points   

QUESTION 10

I have found a digestive enzyme whose optimum pH is 2. Where in the body is this enyzme most likely to function?

stomach

mouth

small intestine

pancreas

beans were carrying out photosynthesis

beans were carrying out fermentation

the solution was becoming more acidic

beans were producing oxygen

Lab 9: Enzymes Concepts to explore: Enzymes -Selectivity . Activators Inhibitors Catalysts . Activation energy .Activation site -Reaction rates Enzymes are specialized proteins that serve as biological catalysts to decrease the activation enerB normally needed for a reaction to occur. This means the reaction rate is up to millions of times faster than it would be without the enzyme. Most biochemical reactions require enzymes for them to occur at fast enough rates to be useful. Typical nomenclature for enzymes follows the pattern using the name of the substrate or the chemical reaction it catalyzes, and ends with ase", eg. catalase, amyl ase. (In other words, any time you see a word end in "ase you know it is an enzyme). Products Enzyme Enzyme Enzyme a.)Suhtrate entering b)Enxyme/suhestrate c) Prodocts leaving active site of enayme active site of enzyme Figure 1: The speciticity of enzymes is controlled by their lock and key fit with a spe- dfic substrate. Enzymes are extremely selective, and are often described as havinga "lock and key" fit (Figure 1). Their shape determines which substrates they bind and interact with. The activation site Lab 9: Enzymes is the pocket where the substrate attaches and where the reaction occurs. After the enzyme/substrate complex forms and catalysils occurs, the "new substrate is released from the active site, and the en zyme can repeat the process. Enzymes levels are not reduced or altered during the reaction. This means they are efficient and can be used repeatedly. Enzymes determine the rate at which the reaction oc rs (not how it occurs). Their activity is affected by temperature, pH, enzyme and substrate concentration, and other chemicals that may be present (such as salts, which can change the protein structure). Variations in temperature and alkalinity can change the shape of the proteins, such as enzymes, which makes them inactive (they can no longer bind to their substrate). The pH can alter charge of the pro

Explanation / Answer

5. The solution has becoming more acidic (bromothymol blue is yellow at acidic pH and green at near neutral pH. When it turns from green to yellow, it shows the medium is becoming acidic)

6. the carbon dioxide produced from respiration combined with water to form carbonic acid, which makes the solution acidic

9. The active sites of the enzymes bind to SUBSTRATES

10. Stomach. It is the organ which has an internal pH below one. However, it rises to 2 or above depending upon the food consumed.

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