enzymes creates unique sticky ends. H H 5 kb 0 kb 1 kb 2 kb 8 kb 10 kb 11 kb PCR

Question

enzymes creates unique sticky ends. H H 5 kb 0 kb 1 kb 2 kb 8 kb 10 kb 11 kb PCR product EcoRI H HindIII N Notl S The plasmid into which you want to clone the p53 gene is shown below. X 10 kb Amp S 1 kb pCDNA3 plasmid B 7 kb 14 kb s 5 kb Part B After cutting the PCR product with Hindlll and Notl, how many pieces of DNA will you have? Enter your answer as a whole number Submit My Answers Give Up Correct When the linear PCR product is cut with the two enzymes, N and H, three pieces of DNA result. The middle piece of DNA--the one between the N site and the first H site--contains the entire p53 gene. Part C After cutting the plasmid with Hindlll and Notl, how many pieces of DNA will you have? Enter your answer as a whole number My Answers Give Up Submit incorrect; Try Again; 4 attempts remaining Part D This question will be shown after you complete previous question(s)

Explanation / Answer

Part c - there is no cutting site present inthe plasmid for Hind 3 and Not1 so it give only 1 fragment or 1 piece.

Related Questions

Navigate

• Browse (All)
• Browse E
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.