1. Calculate the retention factors for the four single component spots shown in

Question

1. Calculate the retention factors for the four single component spots shown in Figure List the measured values of d(s) and the value of d(m) for the four developed spots. Show the calculations. of the developed component spots from a sample had a mobile phase front after development that was 14.9 cm and a component spot that was 2.4 cm from the center of the original sample spot. The paper chromatogram was developed by using the same mobile phase and same paper type that was used to develop the chromatogram shown in Figure 23-1. It is known that the component corresponds to one of the four substances shown in the chromatogram in Figure 23-1. Which of the four substances is the sample component? Show the calculations. 157

Explanation / Answer

For answer of question 1 there should be experimental data or information in book required as from picture it cant be judged the distance component travelled.

Answer 2:

RETENTION FACTOR Rf

Rf= distance spot traveled/distance solvent traveled

Distance is always measured from the spoting line to the spot and again from the spotting line to the solvent front line.

So for given component Rf=2.4/14.9

Rf=0.1610

So after using again the values of answer 1 rest of the answer can be answered.

thanks.

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