1- What are the reaction rates for E1 and E2? 2- what type of graph we should cr

Question

1- What are the reaction rates for E1 and E2?

2- what type of graph we should create for these data?

Remove the blank cuvette from the colorimeter Measure the absorbance for each of your samples using a separate cu each sample. Record the absorbance for each of your samples in Table Table 1: Accumulation of ONP in lactase reactions 1 (El) and 2 (E2) t min A430 E1 o 63 2 10 0.72. 15 Rinse your tubes and cuvettes with distilled water and place them upsid on a clean paper towel next to the computer for later use. VITY 3: Graphing the data (1) edure (Group of 4): Examine your data in table 1. What should be plotted on the x-axis? mo we'll get to that later

Explanation / Answer

Reaction rate for E1 reaction = (final concentration-initial concentration)/(final time-initial time)

                                               = (0.981-0)/(15-0) =0.0654 M/min

E1 is 1st order reaction . You should make a ln[ONP-] vs time graph whhich will give a straight line.

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reaction rate for E2 =(0.72-0)/(15-0) = 0.048 M/min

E2 is second order reaction. You should make 1/[ONP-] vs time plot which will give a straight line

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