You are characterizing a sequence-specific dna binding protein whose molecular w

Question

You are characterizing a sequence-specific dna binding protein whose molecular weight is 20kd, and it ends to one sequence very specifically. You are trying to set up a protein:dna complex such that all the protein in the solution is bound to the dna. You are using a plasmid dna containing 2500 base pairs that has one recognition sequence for this protein. one base pair has a molecular weight of 660 daltons. If you have 20 poles of the protein, how many micrograms of plasmid dna would you need to add to the reaction in order to have all the protein bound to dna?

Explanation / Answer

DNA binding protein molecular weight = 20 Kd

Number of base pairs in plasmid DNA = 2500

Molecular weight of base pair = 660 daltons or 0.66Kd

Number of plasmid DNA required = 2500/20 x 0.66 = 189.33

Thus, 189.33 micrograms of plasmid DNA would required to bound all the protein to DNA.

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