Ok I\'m confused about actin filament polymerization and I havea discussion ques

Question

Ok I'm confused about actin filament polymerization and I havea discussion question that goes like this... Let's pretend you're doing some experiments on actin filamentpolymerization in your kitchen. You are monitoring polymerizationof actin filaments in an in virtro system in which you can specifyand maintain a set concentration of mnomeric ATP- actin in a testtube. You can then follow the polymerization of actin filamentsfrom nucleation through elongation and sometimes, treadmilling.Let's asuume you know from past experience that the criticalconcentraion of + ends in this system is .1microM and the criticalconcentraion of - ends in this system is .6microM 1. you start out with a [ATP-actin] of .4microM A. what is the status of AF growth in this system? B.At a certain point, you run out of ATP-actin and cannot keepfeeding it into the reaction to maintain the .4microMconcentration. What happens to the AFs? C. to add insult to injury, your little brother walks into thekitchen and squirts a 10mM solution of Thymosin he purified frommammalian cells into your test tube. Now what happens to those AFs?(note that you still haven't purchased any new ATP-actin. any suggestions would help, thnx! Ok I'm confused about actin filament polymerization and I havea discussion question that goes like this... Let's pretend you're doing some experiments on actin filamentpolymerization in your kitchen. You are monitoring polymerizationof actin filaments in an in virtro system in which you can specifyand maintain a set concentration of mnomeric ATP- actin in a testtube. You can then follow the polymerization of actin filamentsfrom nucleation through elongation and sometimes, treadmilling.Let's asuume you know from past experience that the criticalconcentraion of + ends in this system is .1microM and the criticalconcentraion of - ends in this system is .6microM 1. you start out with a [ATP-actin] of .4microM A. what is the status of AF growth in this system? B.At a certain point, you run out of ATP-actin and cannot keepfeeding it into the reaction to maintain the .4microMconcentration. What happens to the AFs? C. to add insult to injury, your little brother walks into thekitchen and squirts a 10mM solution of Thymosin he purified frommammalian cells into your test tube. Now what happens to those AFs?(note that you still haven't purchased any new ATP-actin. any suggestions would help, thnx!

Explanation / Answer

Didn't anyone ever tell you not to play with actin polymerization in the kitchen? It's just not sanitary haha jk good questions I have to refresh my mind on the matter a bit but will answer if no one else has in a few days

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