Which of these instruments produces unique fragmentation patterns and works like

Question

Which of these instruments produces unique fragmentation patterns and works like fingerprint for drug identification? a. GLC b. MS c. HPLC d. Raman Spectrometry Among the following which is the least important factors affecting reproducing the Rf values in TLC (Thin Layer Chromatography)? a. Stationary phase b. Temperature c. Mobile phase d. Development distances If an exhibits consists of volatile substances, which of the following instruments can be used to analyze the exhibit? a. GC b. MS d. Capillary Electrophoresis All types of chromatography have: a. Have a stationary phase and a mobile phase b. Have chromatograms with peaks on the chart c. Have a liquid mobile phase d Can be used to separate explosive residue from the debris of an arson The most versatile detector used in GC for the analysis of a substance that contains C-H bond is a) FID b) NPD c) MS d) none of these Pipette, volumetric flask and measuring balance have a. Random error b. Systematic error c. Absolute error d. Standard error Random spectroscopy involves: a. Light absorption b. Scattering of light c. Decomposed light d. Emission of light The IR spectrum of a compound is equivalent to a. Specific in identification b. DNA typing c. Fingerprint d. Partitioning

Explanation / Answer

1) c)HPLC

High performance liquid chromatography(HPLC) is used in drug testing ,mainly for its identification and quantification of their active ingredients by separation of its components using chromatography.

2)b) temperature

Rf=distance travelled by the analyte on the chromatogram/distance travelled by the solvent or the mobile phase

As the distance travelled depends on the binding of the solute and the solvent to the stationary phase. For polar stationary phase like silica gel,polar analyte will have more affinity (due to electrostatic attractions). Analytes having more affinity for the stationary phase travels slowly ,and has lesser distance travelled ,so low Rf.

Temperature does not change the polarity of analyte so is least significant factor in TLC

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