Which of the following is NOT correct when considering the initiation of transcr

Question

Which of the following is NOT correct when considering the initiation of transcription in E. coli?Which of the following is NOT correct when considering the initiation of transcription in E. coli?

A. The RNA polymerase contacts about 50 bp of DNA.
B. Only the -10 region of the promoter is required.
C. The phosphodiester bonds that are formed are not stable until about 10 nucleotides have been incorporated.
D. DNA melting near the transcription start site is essential for promoter recognition.
E. The subunit of RNA polymerase makes strong contacts with the -10 region of promoters.

Explanation / Answer

The incorrect option is

(B) Only the -10 region of the promoter is required.

In fact, the RNA polymerase requires -35 region and -10 region, both of which comprise the core promoter of the prokaryotes. The -35 region is recognized by factor, whereas the core enzyme bind to the -10 site. Overall, the RNA polymerase binds the entire region of the core promoter, from -35 region to -10 region.

(E) The region makes strong contacts with -35 region, whereas the holoenzyme makes contact with the -10 region.

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