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Apyrase Luciferase DNA polymerase ATP sulfurylase Taq polymerase Releases light

ID: 515637 • Letter: A

Question

Apyrase

Luciferase

DNA polymerase

ATP sulfurylase

Taq polymerase

Releases light as a result of luciferin oxidation

Substrate is PPi which is used to create ATP

Creates approximately 106 multiple identical DNA products which are attached to each bead

Synthesizes DNA 5'3'

Eliminates unincorporated nucleotides and ATP

      -       a.       b.       c.       d.       e.   

Apyrase

      -       a.       b.       c.       d.       e.   

Luciferase

      -       a.       b.       c.       d.       e.   

DNA polymerase

      -       a.       b.       c.       d.       e.   

ATP sulfurylase

      -       a.       b.       c.       d.       e.   

Taq polymerase

a.

Releases light as a result of luciferin oxidation

b.

Substrate is PPi which is used to create ATP

c.

Creates approximately 106 multiple identical DNA products which are attached to each bead

d.

Synthesizes DNA 5'3'

e.

Eliminates unincorporated nucleotides and ATP

Explanation / Answer

Ans.

I. Apyrase: e. Eliminated unincorporated nucleotides and ATP.

The enzymes hydrolyze ATP and other NTP into ADP (or, NDP) and AMP (or, NMP) subsequently. Here, N = Nitrogenous base in general. Hydrolysis of nucleotides cause their elimination as energy source from the medium.

II. Luciferase: a. Releases light as a result of luciferin oxidation.

III. DNA polymerase: d. Synthesizes DNA 5’-3-

IV: ATP sulfurylase: b. Substrate is PPi which is used to create ATP

            Sulfate + ATP ----ATP sulfurylase ----> Adenosine 5’-phsophosulfate (APS).

However, the enzyme also catalyzes the backward reaction to produce ATP from PPi and APS as follow-

            APS + PPi ----ATP sulfurylase ----> ATP

V: Taq polymerase: c. Creates approximately 106 multiple identical DNA products which are attached to each bead. This DNA synthesis is carried out in PCR.

Note that DNA polymerase makes only a single copy of each DNA during replication.

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